Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Human TRPV1 is an efficient thermogenetic actuator for chronic neuromodulation

doi: 10.1007/s00018-024-05475-x

Figure Lengend Snippet: Theromogenetic stimulation of neuronal circuits in vivo. A Experimental design. B Targeting the cuneiform nucleus (CnF) with viral intracerebral injections followed by fiber-optic interface implantation. C Experimental setup for simultaneous thermogenetic stimulation using the IR laser and detection of the GCaMP6s calcium sensor signal. Arduino: Arduino controller; BP1 and BP2: bend-pass interference filters; C: multimode collimator; CMOS: videorecorder with CMOS sensor; DM1 and DM2: dichroic mirrors; Fiber: optical fiber; HWP: Zero-Order Half-Wave Plate; L1, L2, and L3: lenses; LED: light-emitting diode with the wavelength of 470 nm; M1 and M2: broadband dielectric mirrors; Obj: objective; PBSC: polarizing beamsplitter cube. D IR-laser stimulation patterns. Heating periods when the IR laser was on were commonly 20 s. If a mouse ran to too fast, the IR laser was switched off earlier than the 20-s period over. E The average speed of mice within the 30 s period prior to the first application of the heating IR laser on the first day of thermal stimulation. Quantitative data are shown as mean ± SEM. Statistics: Student’s t -test. p = 0.5198. t = 0.6616. F = 2.066. hTRPV1⁺: N = 7 mice. Control: N = 8 mice. ns: not significant. F and G Thermogenetic stimulation of excitatory neurons in CnF evokes locomotion and elevates the GCaMP6s signal in hTRPV1⁺ mice ( F ), but not in control mice ( G ). IR laser power was 40 mW. Graphs indicate representative curves of motion speed (blue) and integral intensity of the GCaMP6s signal (vermillion) in hTRPV1⁺ and control mice during periods when the IR-laser was on or off (yellow). See also Supplementary Video

Article Snippet: The optical stimulation/photometry setup consisted of the following components (Fig. C): 1342 nm: IR continuous wave laser with wavelength 1342 nm (CNI, MIL-III-1342-1W); Arduino: Arduino controller; BP1 and BP2: band pass filter (Thorlabs, MF525-39 and Chroma, ET480/20x); C: collimator (Thorlabs, F950FC-A); Camera: CMOS camera (Thorlabs, CC505MU); CMOS: CMOS camera (The Imaging Source, DMK 33UX226); DM1 and DM2: dichroic mirror (Thorlabs, DMLP490 and DMSP900); Fiber: optical fiber with core diameter 200 μm and NA = 0.22 (Thorlabs, FG200LEA); HWP: half wave plate (Thorlabs, WPH05M-1310); L1, L2, and L3: spherical lenses (Thorlabs, LSA01); LED: fiber coupled light emitting diode with central wavelength 470 nm (Thorlabs, M470F4) with a fiber (Thorlabs, M134L02); M1 and M2—dielectric mirror (Thorlabs, BB1-E02 and BB1-E04); Obj: microscope objective (Mitutoyo,10X Mitutoyo Plan Apochromat Objective, 480–1800 nm, NA = 0.26, WD = 30.5 mm, MY10X-823); PBSC: Polarizing Beamsplitter Cube (Thorlabs, PBS124).

Techniques: In Vivo, Control

Journal: Sensors (Basel, Switzerland)

Article Title: The Evaluation of a Low-Cost Colorimeter for Glucose Detection in Salivary Samples

doi: 10.3390/s17112495

Figure Lengend Snippet: Average cost of colorimeter development.

Article Snippet: The Arduino UNO, RGB module (ISL29125) and Real Time Clock (RTC) module (TDLH02) were supplied from Sparkfun Electronics (Niwot, CO, USA).

Techniques:

Journal: Sensors (Basel, Switzerland)

Article Title: New Approaches to Implementing the SmartJacket into Industry 4.0 ‡

doi: 10.3390/s19071592

Figure Lengend Snippet: ( a ) An Arduino LilyPad and the wire connection of the sensors; ( b ) An MQ-135 air quality sensor; ( c ) An HC-SR-04 ultrasonic sensor; ( d ) a DS18B20 1-wire temperature sensor.

Article Snippet: The LilyPad Arduino was designed and developed by Leah Buechley and SparkFun Electronics (Niwot, CO, USA).

Techniques: